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INS-1 832/3 cells lacking endogenous GLP-1R after deletion by CRISPR/Cas9 (Naylor et al., 2016) (a gift from Dr Jacqueline Naylor, MedImmune) were used to generate a polyclonal population expressing human SNAP-GLP-1R by G418 selection, and were maintained as for wild-type INS-1 832/3 cells, with the addition of G418 (1 mg/ml)

It may be reasonable to continue carnitine until the patient has improved clinically, valproate concentrations are at a therapeutic level, and ammonia levels have normalized

Cells lacking both SLC25A39 and its paralog SLC25A40 exhibit defects in the activity and stability of ironsulfur cluster containing proteins

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